Data from the paper Shalem O*, Sanjana N* et al. Science 2013

  1. Normalized read counts from A375 screen: norm_read_count_A375.txt
  2. Depletion data for A375 and HUES62: depletion_data

Description of each data set:

  1. Contains normalized read counts for the different sgRNAs in 5 time points:
    • Plasmid pool sequencing: before virus production
    • DMSO and PLX after 7 and 14 days of treatment post puromycin selection
  2. For each gene the mean sgRNA log 2 ratio between to time points: 3 days (right after infection) and 14 days post puromycin selection

Please feel free to contact us for any questions related to this data:

Ophir Shalem (oshalem et

Neville Sanjana (neville et

Feng Zhang (zhang et