Vector maps


lentiCRISPRv2 (available on Addgene): Single vector with Cas9 and sgRNA on same virus, plasmid map on benchling in GenBank and FASTA formats)

lentiCRISPRv2 map


lentiCas9-Blast (Addgene) & lentiGuide-Puro (Addgene): Two vector with Cas9 and sgRNA delivered as separate viruses, plasmid maps on benchling for lentiCas9-Blast & lentiGuide-Puro in GenBank and FASTA formats)

LentiCas9-Blast map

lentiGuide-Puro map


Legacy lentiCRISPRv1 (no longer available, please use lentiCRISPRv2 which has 10X higher titer): Single vector with Cas9 and sgRNA (as used in Shalem*, Sanjana*, et al., Science, 2014). Plasmid map on benchling.

lentiCRISPR vector map